Composite

Part:BBa_K2036026:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)


PP2CA-TTADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PP2CA can inactivate ABF2 or other related transcriptional activator in plants response element.


Source

From Arabidopsis thaliana

References

Tim Lynch • B. Joy Erickson • Ruth R. Finkelstein(2012) Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response. Plant Mol Biol. 2012 Dec;80(6):647-58. doi: 10.1007/s11103-012-9973-3. Epub 2012 Sep 25.
Fen-Fen Soon, Ley-Moy Ng, X. Edward Zhou, Graham M. West, Amanda Kovach, M. H. Eileen Tan, Kelly M. Suino-Powell,Yuanzheng He, Yong Xu, Michael J. Chalmers, Joseph S. Brunzelle, Huiming Zhang（2012）Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases. Science. 2012 Jan 6;335(6064):85-8. doi: 10.1126/science.1215106. Epub 2011 Nov 24.